Figure 1: The Agilent 2100 Bioanalyzer.
Figure 2: The Agilent 2100 Bioanalyzer.
Dr. Allison Gillaspy: 405-271-2337 ext. 1
Agilent 2100 Bioanalyzer: RNA, DNA and Protein Sample Analysis
The Agilent 2100 Bioanalyzer is a microfluids-based platform replacing standard gel electrophoresis for RNA sample QC, DNA fragment analysis, and SDS-page electrophoresis for proteins.
The small volume allows you to conserve valuable sample and with a 30 minute run-time per chip you will be able to determine the quality and quantity of up to 12 total RNA samples and 11 small RNA samples. The analysis will include graphic visualization of ribosomal peaks (23S and 16S) or (28S and 18S), ribosomal ratio, RNA concentration, a calculated RIN (RNA Integrity Number) value, and a gel-like image RNA sample. Although the Bioanalyzer analysis will quantitate RNA samples, in our experience RNA readings are more accurate using the NanoDrop ND-1000 UV-Vis Spectrophotometer which we provide for each sample submitted. NanoDrop is also available as a separate service for both RNA, DNA, and protein quantitation.
The following is a partial list of applications for Agilent's Lab-on-a-Chip® technology for RNA, DNA, and protein analysis. For a more complete list of applications to meet your research needs: Agilent Bioanalyzer Compendeum. At this time the Bioanalyzer 2100 in the core facility is not equipped with a flow cell needed for cell fluorescence analysis.
For our customers that wish to view the actual Bioanalyzer results, the latest software version B.02.05 is freely available to download in the demo version from the Agilent website. Use the following instructions to help guide you through the process: Agilent Bioanalyzer software download instructions.
We currently have chips capable of analyzing RNA in nanogram concentrations. For analysis of sample RNA in the picogram range and for DNA or protein applications, please contact us so we can help you determine the appropriate chip for your research.