Instructions for macroarray printing submissions

PLEASE NOTE: Due to continuing concerns posed by potential bioterrorism agents (select agents), our online submission form requires that you provide us with information on the original biological source of any DNA from which you will be asking us to obtain sequence. "Original biological source" refers to the organism from which the DNA was first found in nature (human, HIV, Staphylococcus aureus, etc.). If you simply put a nonspecific term such as "E. coli clone", this will not be sufficient. We realize that adding yet another field to fill out on these forms can be tiresome, but this information is necessary to protect our staff against potential legal difficulties. Thank you very much for your understanding.

            - David W. Dyer, Ph.D.

 

 requirements

• Samples must be submitted in 96-well V-bottom plates (minimum volume of 10 µl necessary)
• 1 to 4 plates can be printed onto one macroarray membrane.
• Samples are suggested to be normalized (usually to 100 ng/µl) before spotting. If a customer needs this service, an extra fee will be added to each plate.

 

 additional notes

• Plasmid DNA, PCR products or genomic DNA can be printed on membrane arrays.
• All arrays are printed on positively charged nylon Amersham membranes in duplicate, in a 4x4 arrangement allowing for a maximum of 768 spots.
• If samples are submitted in plates other than V-bottom the customer will also be charged for the use of V-bottom plates as well as the tips and labor involved in sample transfer.
• The membranes will be UV crosslinked to fix the DNA to the membrane and then stored in plastic wrap and foil.
• Customers will be contacted via e-mail upon array completion so they can pick up the finished arrays.

 

 form

macroarray printing submission form