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OUHSC Laboratory for Genomics and
Bioinformatics Fee Structure |
Updated 07-28-08 |
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DNA Sequencing, Fragment Analysis and Genotyping |
Price |
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Small-Scale Custom
DNA Sequencing (Academic) For submissions of <96 per plate |
$6.00/reaction |
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Large Scale Custom
DNA Sequencing (Academic) For submissions of full 96-well plates |
$4.00/reaction |
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Whole Genome
Sequencing and Library Construction |
Contact us |
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Small-Scale
Fragment Analysis (microsatellite, AFLP, TRFLP and SNP) Rate applies to submissions of less than 96 samples at a time of fluorescently labeled products for size or allele determination using the ABI 3730xl. Fragment lengths can range from 35bp to 600bp for microsatellite samples and 15bp to 120bp for SNP samples. *Please contact us directly if you require facility personnel to perform amplification with fluorescent primers as pricing will be affected. |
$5.00/sample |
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High Throughput
Fragment Analysis (microsatellite, AFLP, TRFLP and SNP) Rate applies to submissions of 96 samples at a time of fluorescently labeled PCR products for size or allele determination using the ABI 3730xl. Fragment lengths can range from 35bp to 600bp for microsatellite samples and 15bp to 120bp for SNP samples. *Please contact us directly if you require facility
personnel to perform amplification with fluorescent primers as pricing will be
affected. |
$3.00/sample |
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Sample clean up and quantitation |
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SAP/ExoI treatment For cleanup of PCR products prior to cycle sequencing. Samples should contain a single product as verified on an agarose gel prior to submission. |
$1.00/reaction |
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Spec reading of DNA
in 96 well plate Absorbance read at 260 and 280 using Molecular Devices SpectraMax. Concentration and 260/280 ratios provided for each sample. |
$11.00/plate |
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Nanodrop: RNA and DNA Quantitation (up to 10 samples) The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis. Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms. |
$3.00 |
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Nanodrop: RNA and DNA Quantitation (11-20 samples) The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis. Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms. |
$5.00 |
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Nanodrop: Protein Quantitation
with standard curve (up to 10 samples) Facility personnel will perform BCA protein assay on
sample and will perform standard curve. |
$45.00 |
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Nanodrop: Protein Quantitation
with customer provided standard curve (up to 10 samples) Customer must provide standard curve information or
standards for reading on Nanodrop. Pricing is the same as for RNA and DNA quantitation. |
$3.00 |
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Bioanalyzer: RNA, DNA, and Protein Analysis (1-12
samples/run) To determine quality of sample prior to use in downstream applications such as microarrays, real time PCR, sequencing, and binding assays. *RNA analysis available for either total RNA or small RNA (≤100bp). Small RNA chip holds only 11 samples. |
$40.00/run |
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Other Related Services |
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DNA normalization Includes spec reading (A260/280) of samples in plate and normalization to constant volume and concentration across plate. |
$15.00/plate |
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Transfer of DNA
using Biomek NX Transfer of equal volumes for each DNA sample from 96-well plate to a 96- or 384- well plate. Price for transfer of samples to 384-well is up to four 96-well plates per one 384-well plate. Used for setting up PCR, Real Time and sequencing reactions in bulk. Transfer of equal volumes of sample from 96- or 384- well plate to multiple 96- or 384- well plates. Price given is for transfer to four 384-well plates. *Costs may vary slightly based on plate
type needed. If multiple transfers are done at the same time, difference in
charge is based on cost of additional plates. |
$25.00/plate $45.00 |
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High Throughput
Culture and Plasmid Prep Includes inoculation of 96-well deep well block with cells from freezer stock provided by customer. Freezer stock of re-growth provided with isolated DNA from Millipore Montage 96-well plasmid prep. *NOTE: Cost for
prepping and immediate sequence of clones in one direction is cheaper than
sequencing at a later time. |
$2.00/well w/o seq. $5.00/well includes first plate of sequences |
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96 well freezer
stock -80ºC freezer stock of 96-well bacterial cultures provided in sterile, flat bottom plate with final concentration of glycerol 25%. |
$6.50/plate |
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High throughput PCR 96-well PCR (25µl reactions) using the same primer pair for each sample. Cost includes use of any of the facility provided universal primers listed on the sequencing section of our webpage. * If custom primers are used then they must be added by the customer prior to cycling. DNA template must be provided in 96- or 384- well format. |
$100/plate $1.05/sample (min. 48 samples) |
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PF2D and Related Services |
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PF2D Protein
Fractionation (1st and 2nd dimensions) Complete run of one sample that requires lysis and complete fractionation. Price includes all 96-well deep well blocks for storage of 1st and 2nd dimension fractions. |
$700/sample |
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1st
dimension run with sample prep and assay Available for customers that require only isoelectric focusing of whole cell lysates. Both lysis and BCA protein assay included as well as single 96-well deep well block. |
$375/sample |
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1st
dimension run only without sample prep Available for customers that require isoelectric focusing of samples such as purified protein mixtures or spent supernate. |
$315/sample |
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Single fraction run
through 2nd dimension only Can be run to collect additional protein from previous 1st dimension run or for lower complexity samples that do not require 1st dimension in order to examine hydrophobicity profiles. |
$30.00/run |
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Sample Prep &
BCA Protein Assay |
$60.00/sample |
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BCA Protein Assay |
$50.00/sample |
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Real Time PCR and related services |
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TaqMan Universal PCR Master Mix |
$2.50/reaction |
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TaqMan Fast Universal PCR Master Mix |
$1.00/reaction |
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SYBR Green Master
Mix or POWER SYBR Green |
$1.00/reaction |
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Fast SYBR Green
Master Mix |
$1.00/reaction |
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*Services listed above reflect costs for facility
personnel to add master mix to all wells and to cycle on ABI7500. Customer provides sample and primers only
in plate. |
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Standard Mode
cycling only Includes allelic discrimination end point scan if necessary. |
$3.50/plate |
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Fast Mode cycling
only |
$1.50/plate |
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*Services listed above valid only when
customer provides samples in optical plate with master mix and all other
components added. |
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Allelic
discrimination end point scan *For plates that
have been set up and cycled by customer. |
$0.50/plate scanned |
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Array Services |
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Promega Improm-II cDNA synthesis *Synthesis using customer supplied, purified RNA sample. cDNA for use in Real Time with SYBR Green reagents or for Microarray experiments using any dye set. |
$4.50/reaction |
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cDNA cleanup |
$3.00/reaction |
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RNA cleanup Includes DNAse treatment and further purification of RNA sample prior to cDNA synthesis. |
$6.00/reaction |
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Microarray
Processing Includes labeling, hybridization and scanning. Customer provides microarray and purified RNA at appropriate concentration. *All array types accepted (includes glass slides, Affymetrix, Combimatrix, etc). |
Contact us |
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RNA isolation for
microarray |
Contact us |
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Microarray scanning
only (glass slides or Combimatrix arrays) Appointment should be made in advance. Scanners used are Perkin Elmer HT Scan Array (glass slides) or Combimatrix ElectraSense (detects fluorescent dyes and chemiluminescence on Combimatrix chip). |
$20.00/slide |
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Macroarray printing on nylon membranes Up to 8 plates of 96 samples can be spotted in duplicate per membrane using a 4x4 array format. |
$15.00/membrane |
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Bioinformatics
Support Includes analysis of array data using GeneSpring software suite, batch analysis of DNA sequences (vector trimming, blast, assembly) large scale primer design, database design and custom script development. *Additional services available. Please inquire. |
$75.00/hour |
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BiaCore Services |
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BiaCore Analysis Includes analysis of binding interactions of ligand and multiple analytes using a single chip. BiaCore technology can be used to analyze interactions such as protein:protein, DNA:protein, DNA:RNA. Customer purchases chip direct from company or from the facility. Buffers, tubes, and other consumables necessary for experiment are included in cost. |
$40.00/hour |
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BiaCore
Consultation This service is available if additional experimental design by the facility is necessary or if consultation prior to experiment is required. Experienced BiaCore customers (or return customers) may not require this service. |
$75.00/hour |