Real Time PCR
TaqMan Universal PCR Master Mix	$2.50/reaction
TaqMan Fast Universal PCR Master Mix	$2.00/reaction
SYBR Green Master Mix or POWER SYBR Green	$2.00/reaction
Fast SYBR Green Master Mix	$2.00/reaction
*Services listed above reflect costs for facility personnel to add master mix to all wells and to cycle on ABI7500. Customer provides template and primers in plate.
Standard Mode cycling only Includes allelic discrimination end point scan if necessary.	$3.50/plate
Fast Mode cycling only	$1.50/plate
*Services listed above valid only when customer provides samples in optical plate with master mix and all other components added.
Allelic discrimination end point scan *For plates that have been set up and cycled by customer.	$0.50/plate scanned
Other Related Services
Nanodrop: RNA and DNA Quantitation (up to 10 samples) The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis. Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms.	$3.00
Nanodrop: RNA and DNA Quantitation (11-20 samples) The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis. Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms.	$5.00
Nanodrop: Protein Quantitation with standard curve (up to 10 samples) Facility personnel will perform BCA protein assay on sample and will perform standard curve.	$45.00
Nanodrop: Protein Quantitation with customer provided standard curve (up to 10 samples) Customer must provide standard curve information or standards for reading on Nanodrop. Pricing is the same as for RNA and DNA quantitation.	$3.00
Bioanalyzer: RNA, DNA, and Protein Analysis (1-12 samples/run) To determine quality of sample prior to use in downstream applications such as microarrays, real time PCR, sequencing, and binding assays. *RNA analysis available for either total RNA or small RNA (≤100bp). Small RNA chip holds only 11 samples.	$40.00/run
Transfer of DNA using Biomek NX Transfer of equal volumes for each DNA sample from 96-well plate to a 96- or 384- well plate. Price for transfer of samples to 384-well is up to four 96-well plates per one 384-well plate. Used for setting up PCR, Real Time and sequencing reactions in bulk. Transfer of equal volumes of sample from 96- or 384- well plate to multiple 96- or 384- well plates. Price given is for transfer to four 384-well plates. *Costs may vary slightly based on plate type needed. If multiple transfers are done at the same time, difference in charge is based on cost of additional plates.	$25.00/plate $45.00