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Real Time PCR |
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TaqMan Universal PCR Master Mix |
$2.50/reaction |
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TaqMan Fast Universal PCR Master Mix |
$1.00/reaction |
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SYBR Green Master
Mix or POWER SYBR Green |
$1.00/reaction |
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Fast SYBR Green Master Mix |
$1.00/reaction |
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*Services listed above reflect costs for
facility personnel to add master mix to all wells and to cycle on
ABI7500. Customer provides sample and
primers only in plate. |
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Standard Mode
cycling only Includes allelic discrimination end point scan if necessary. |
$3.50/plate |
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Fast Mode cycling
only |
$1.50/plate |
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*Services listed above valid only when
customer provides samples in optical plate with master mix and all other
components added. |
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Allelic
discrimination end point scan *For plates that
have been set up and cycled by customer. |
$0.50/plate scanned |
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Other Related Services |
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Nanodrop: RNA and DNA Quantitation (up to 10 samples) The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis. Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms. |
$3.00 |
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Nanodrop: RNA and DNA Quantitation (11-20 samples) The Nanodrop uses undiluted sample in a minimum volume of 1µl and a maximum volume of 2µl which can be recovered after analysis. Sensitivity range is from 5ng to 3700ng, making the Nanodrop useful for determining concentration of samples that may be limited in volume or that may be too dilute for accurate reading on other platforms. |
$5.00 |
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Nanodrop: Protein Quantitation
with standard curve (up to 10 samples) Facility personnel will perform BCA protein assay on
sample and will perform standard curve. |
$45.00 |
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Nanodrop: Protein Quantitation
with customer provided standard curve (up to 10 samples) Customer must provide standard curve information or
standards for reading on Nanodrop. Pricing is the same as for RNA and DNA quantitation. |
$3.00 |
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Bioanalyzer: RNA, DNA, and Protein Analysis (1-12
samples/run) To determine quality of sample prior to use in downstream applications such as microarrays, real time PCR, sequencing, and binding assays. *RNA analysis available for either total RNA or small RNA (≤100bp). Small RNA chip holds only 11 samples. |
$40.00/run |
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Promega Improm-II cDNA synthesis *Synthesis using customer supplied, purified RNA sample. cDNA for use in Real Time with SYBR Green reagents or for Microarray experiments using any dye set. |
$4.50/reaction |
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cDNA cleanup |
$3.00/reaction |
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RNA cleanup Includes DNAse treatment and further purification of RNA sample prior to cDNA synthesis. |
$6.00/reaction |
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Microarray
Processing Includes labeling, hybridization and scanning. Customer provides microarray and purified RNA at appropriate concentration. *All array types accepted (includes glass slides, Affymetrix, Combimatrix, etc). |
Contact us |
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RNA isolation for
microarray |
Contact us |
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Microarray scanning
only (glass slides or Combimatrix arrays) Appointment should be made in advance. Scanners used are Perkin Elmer HT Scan Array (glass slides) or Combimatrix ElectraSense (detects fluorescent dyes and chemiluminescence on Combimatrix chip). |
$20.00/slide |
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Macroarray printing on nylon membranes Up to 8 plates of 96 samples can be spotted in duplicate per membrane using a 4x4 array format. |
$15.00/membrane |
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Bioinformatics
Support Includes analysis of array data using GeneSpring software suite, batch analysis of DNA sequences (vector trimming, blast, assembly) large scale primer design, database design and custom script development. *Additional services available. Please inquire. |
$75.00/hour |
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Transfer of DNA
using Biomek NX Transfer of equal volumes for each DNA sample from 96-well plate to a 96- or 384- well plate. Price for transfer of samples to 384-well is up to four 96-well plates per one 384-well plate. Used for setting up PCR, Real Time and sequencing reactions in bulk. Transfer of equal volumes of sample from 96- or 384- well plate to multiple 96- or 384- well plates. Price given is for transfer to four 384-well plates. *Costs may vary slightly based on plate
type needed. If multiple transfers are done at the same time, difference in
charge is based on cost of additional plates. |
$25.00/plate $45.00 |