CHEF Map comparison
CHEF Map comparison of S. aureus strains 8325, COL and 6390
Restriction size table:
Figure 5. Circular map of Staphylococcus aureus NCTC 8325 with ORFs color-coded by function.
Staphylococcus aureus NCTC 8325
This work was supported by USPHS grant R01-AI43568 to Dr. Iandolo from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. and by a grant from the Merck Genome Research Institute. Annotation and organization of the genome in Dr. Iandolo's lab is being supervised by Dr. Veronica Worrell. Shotgun sequencing was carried out by sub-contract to Dr. Bruce Roe at the Advanced Center for Genomic Technology at the University of Oklahoma in Norman. The sequencing team in Dr. Roe's lab was led by Yudong Qian and Angela Dorman.
The Principal Investigator on this project is Dr. John Iandolo, Chair, Department of Microbiology and Immunology at OUHSC. Sequencing and assembly of the 2.8 megabase genome of Staphylococcus aureus 8325 is complete and the genome has been circularized. We are in the process of annotation, but the entire sequence can be downloaded at any time.
Strain 8325 is generally regarded as the prototypical strain for all genetic manipulation. Overall, its chromosomal organization is similar to that of the genomes of strains COL (sequenced by TIGR) and strains N315 and Mu50 sequenced by the Hiramatsu group. Differences discovered thus far are due to the presence of various mobile elements, plasmids and phage.
Strain 8325 contains 3 temperate bacteriophages, ø11, ø12 and ø13. The sequences of the phages have been extracted and annotated. These sequences and physical maps of the genomes are available on this site. These phages are compatible and although of dissimilar sequence are genetically organized in similar modular components. Phage ø13 is a converting phage and inserts into the ß-toxin gene, inactivating it. However, it also carries the gene for staphylokinase (sak) and is responsible for introduction of that phenotype in strain 8325. The att-site for ø12 also is within a gene whose function is unknown. The ø11 att-site is located in an inter-genic region.
Other Staphylococcus aureus strain sequencing projects
This work was supported by a grant from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. and the Merck Genome Research Institue.
Figure 1. Pulse field generated restriction map of S. aureus 8325. Click image for full size version.
Figure 2. Composite 50 codon ORF map overlaid on the restriction and functional
maps of ø11.
Figure 3. Composite 50 codon ORF map overlaid on the restriction map of ø12.