CHEF Map comparison

CHEF Map comparison of S. aureus strains 8325, COL and 6390

Restriction size table:

Frag- ment SmaI CspI SgrAI AscI
A 674 688 835 828
B 361 527 418 527
C 324 513 403 423
D 262 249 352 381
E 257 205 183 293
F 208 190 176 132
G 175 154 88  
H 135 66 73  
I 117 59 66  
J 80      
K 76      
L 44      
M 36      
N 20      
O 17      
P 9      
Q 3      
R        

 

 Figure 5

Figure 5. Circular map of Staphylococcus aureus NCTC 8325 with ORFs color-coded by function.

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  • post-script version
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     Figure 6

    Figure 6. Linear map of Staphylococcus aureus NCTC 8325 with ORFs color-coded by function.

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  • post-script version
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     Staphylococcus aureus NCTC 8325

    This work was supported by USPHS grant R01-AI43568 to Dr. Iandolo from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. and by a grant from the Merck Genome Research Institute. Annotation and organization of the genome in Dr. Iandolo's lab is being supervised by Dr. Veronica Worrell. Shotgun sequencing was carried out by sub-contract to Dr. Bruce Roe at the Advanced Center for Genomic Technology at the University of Oklahoma in Norman. The sequencing team in Dr. Roe's lab was led by Yudong Qian and Angela Dorman.

    The Principal Investigator on this project is Dr. John Iandolo, Chair, Department of Microbiology and Immunology at OUHSC. Sequencing and assembly of the 2.8 megabase genome of Staphylococcus aureus 8325 is complete and the genome has been circularized. We are in the process of annotation, but the entire sequence can be downloaded at any time.

    Strain 8325 is generally regarded as the prototypical strain for all genetic manipulation. Overall, its chromosomal organization is similar to that of the genomes of strains COL (sequenced by TIGR) and strains N315 and Mu50 sequenced by the Hiramatsu group. Differences discovered thus far are due to the presence of various mobile elements, plasmids and phage.

    Strain 8325 contains 3 temperate bacteriophages, 11, 12 and 13. The sequences of the phages have been extracted and annotated. These sequences and physical maps of the genomes are available on this site. These phages are compatible and although of dissimilar sequence are genetically organized in similar modular components. Phage 13 is a converting phage and inserts into the -toxin gene, inactivating it. However, it also carries the gene for staphylokinase (sak) and is responsible for introduction of that phenotype in strain 8325. The att-site for 12 also is within a gene whose function is unknown. The 11 att-site is located in an inter-genic region.

     

     Other Staphylococcus aureus strain sequencing projects

    • TIGR's Comprehensive Microbial Resource (CMR) is a tool that allows researchers access to many bacterial genome sequences completed to date. For each genome not sequenced at TIGR two kinds of annotation are displayed: the Primary annotation taken from the genome sequencing center and the TIGR annotation generated by an automated annotation process at TIGR. Among the available genome sequences are those for 3 Staphylococcus aureus strains, COL, Mu50, and N315.
    • The Sanger Centre is sequencing 2 additional strains of S. aureus, the epidemic MRSA strain EMRSA-16, and a hyper-virulent community acquired MSSA strain 476. Click here to visit their S. aureus site.

     

     Data access

  • BLAST the S. aureus genome
  • Download S. aureus genome sequence
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     Annotation

  • Download the S. aureus strain NCTC 8325 annotation list of ORFs with assigned function.
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     Acknowledgements

    This work was supported by a grant from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. and the Merck Genome Research Institue.

     

     Figure 1

    Figure 1. Pulse field generated restriction map of S. aureus 8325. Click image for full size version.

     

     Figure 2

    Figure 2. Composite 50 codon ORF map overlaid on the restriction and functional maps of 11.

  • full view
  • genome sequence
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     Figure 3

    Figure 3. Composite 50 codon ORF map overlaid on the restriction map of 12.

  • full view
  • genome sequence
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     Figure 4

    Figure 4. Composite 50 codon ORF map overlaid on the restriction map of 13.

  • full view
  • genome sequence
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