Streptococcus pyogenes SF370 (M1 serotype)
The Genomic Sequence of Streptococcus pyogenes SF370 (M1 serotype) has been completed through a joint effort between the laboratory of Dr. Joseph Ferretti in the Department of Microbiology and Immunology at the University of Oklahoma Health Sciences Center and Dr. Bruce Roe at the University of Oklahoma, Norman Campus's Advanced Center for Genome Technology
The 1,852,442 base pair sequence of an M1 strain of Streptococcus pyogenes, a Gram-positive pathogen, has been determined and contains 1,752 protein-encoding genes. Approximately one-third of these genes have no identifiable function, with the remainder falling into previously characterized categories of known microbial function. Consistent with the observation that S. pyogenes is responsible for a wider variety of human disease than any other bacterial species, more than forty virulence-associated genes have been identified. Additional new genes have been identified that encode proteins likely associated with microbial "molecular mimicry" of host characteristics and involved in rheumatic fever or acute glomerulonephritis. The complete or partial sequence of four different bacteriophage genomes is also present, with each containing genes for one or more new superantigen-like proteins. These prophage associated genes encode at least six new potential virulence factors emphasizing the importance of bacteriophages in horizontal gene transfer and a possible mechanism for generating new strains with increased pathogenic potential.
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This work was supported by a grant from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. We are appreciative of the assistance in annotation by Gerry Myers and Thomas Brettin of the Los Alamos National Laboratory, Los Alamos, NM. We thank Horst Malke, Vincent Fischetti, Michael S. Gilmore, and David W. Dyer for helpful discussions and comments; and Carolyn Thompson, Min Zhan, RunYing Tian, C. A. Reece and Linda Ray for technical assistance.
Circular map of Streptococcus pyogenes from:"Complete genome sequence of an M1 strain of Streptococcus pyogenes" - PNAS 98:8 p4658